The first step was to protect the amino group of Tris by using t-butyloxycarbonyl anhydride(( Boc) 2O), then mPEG was activated by changing hydroxide terminal into acid chloride and carboxylation of mPEG was fulfilled before. 利用叔丁氧羰基酸酐((Boc)2O)对Tris上的氨基进行保护,对mPEG端羟基依次进行羧基化、酰氯化等活化处理,通过酰氯与羟基的官能团反应合成出目标分子。
In a conclusion, E. coli T protein do have independent domains, N terminal 93 amino acids belongs to chorismate mutase and C terminal 277 amino acids belongs to prephenate dehydrogenase 研究表明,大肠杆菌T蛋白由两个独立结构域组成,N端93个氨基酸组成了分支酸变位酶,C端277个氨基酸组成了预苯酸脱氢酶
Cytosolic precursor, the pre EPSP synthase, becomes mature EPSP synthase after a cut process of the amino terminal transit peptide of cytosolic precursor in the chloroplast. 高等植物前体EPSP合成酶进入叶绿体后,经剪切而成成熟EPSP合成酶并定位于叶绿体内质膜上;
Methods: Various analytical techniques including gene sequencing, amino acid composition, analysis of N terminal amino acid analysis, peptide mapping, IEF, molecular weight and UV scanning were used. 方法:用基因测序、氨基酸组成分析、N末端氨基酸测序、胰肽图谱、IEF、分子量、紫外扫描等各种实验手段对重组L天冬酰胺酶及其天然品进行了全面比较。
Dietary Cellulose's Effect on the Flow of Amino Acid and Endogenous Amino Acid at the Terminal Ileum of Pig 日粮纤维对猪回肠氨基酸及其内源性氨基酸流量的影响
It had a signal peptide with 27 amino acid residues in N-terminal. 它的N-末端存在一个长度为27个氨基酸残基的信号肽。
Clinical value of amino-terminal pro brain natriuretic peptide in pediatric cardiology 氨基末端脑钠尿肽激素原对小儿心功能的评估
Conclusion When fusion protein is purified by Ni2+ affinity chromatography, the position of 6-His tag is very important for the purification of fusion protein. 6-His tag could be placed at the amino terminal and carboxy terminal of fusion protein except the middle. 结论应用镍亲和层析的方法纯化融合蛋白时,His-tag在融合蛋白中的位置对蛋白质的纯化非常重要,His-tag可以在融合蛋白的N端和C端,但不能在中间。
Influence of different pH and sealing of its amino terminal on the anti-endotoxin effect of glycine 不同pH及封闭氨基端对甘氨酸拮抗内毒素作用的影响
A study on measurement of endogenous amino acids flow at the terminal ileum of pigs using the homoarginine method 老年人内源性葡萄膜炎高精氨酸法测定猪回肠内源性氨基酸排泄量的研究
There was an identical frame-shift mutation resulting in changes of 19 amino acids near the 3 ′-terminal domain of the 3 strains each. 它们均在近3′端有一段相同的移码突变,造成19个氨基酸的完全改变。
The protective effects of recombinant amino terminal fragment of bactericidal/ permeability increasing protein on systemic hemodynamics and microcirculation in a rat model of endotoxic shock 杀菌/通透性增加蛋白对内毒素休克大鼠微循环灌注的保护作用
Methods: A fusing expressed plasmid, which contains the 106 amino acids at the carboxyl terminal of APP, was constructed. Screening against human embryo brain cDNA library by two hybrid system was performed. 方法:构建含有APP羧基端106个氨基酸的融合表达质粒,用酵母双杂交系统筛选人胎脑cDNA文库。
OBJECTIVE: To study on the synthesis of galactopyranoside derivatives having an amino group at the aglycon terminal. 目的:研究配基部分含氨基的半乳糖苷衍生物的合成。
Results The three dimensional structure of SED, similar to that of other enterotoxin superantigens, was composed of two domains: amino terminal domain and carboxyl terminal domain. 结果SED的空间结构与其它肠毒素超抗原相似,具有两个结构域:氨基末端结构域和羧基末端结构域。
Purpose To determine the molecular weight, isoelectric point ( pI), amino acid compositions and N terminal amino acid sequence of streptococcus mitis pyrogenic exotoxin ( SMPE). 目的研究缓症链球菌外毒素蛋白的分子量、等电点、氨基酸组成及N末端序列。
To determine the number average molecular mass of chitosan-oligosaccharide by u-sing the acetylacetone's method based on the color-producing reaction with amino terminal. 采用乙酰丙酮与氨基端反应的生色原理,将乙酰丙酮法应用于甲壳胺寡糖数均分子量的测定。
The inhibitor of apoptosis protein ( IAP) family is a sort of important factors of inhibiting apoptosis, which is characterized by the presence of one or more baculoviral IAP repeat domains at the amino terminal, possessing or not a carboxyl terminal RING zinc-finger domain. 凋亡蛋白抑制因子家族是一类重要的抗细胞凋亡因子,该家族结构的共同特点是N端含有一个或多个串联的杆状病毒IAP重复序列,C端含或不含有一个环指结构。